Mouse Thrombomodulin ELISA Kit PicoKine®

• Background:

  Thrombomodulin (TM), CD141 or BDCA-3 is an integral membrane protein expressed on the surface of endothelial cells and serves as a cofactor for thrombin. It reduces blood coagulation by converting thrombin to an anticoagulant enzyme from a procoagulant enzyme. Thrombomodulin is also expressed on human mesothelial cell, monocyte and a dendritic cell subset. In humans, thrombomodulin is encoded by the THBD gene. The protein has a molecular mass of 74kDa, and consists of a single chain with 5 distinct domains. Thrombomodulin functions as a cofactor in the thrombin-induced activation of protein C in the anticoagulant pathway by forming a 1:1 stoichiometric complex with thrombin. This raises the speed of protein C activation thousandfold. Thrombomodulin-bound thrombin has procoagulant effect at the same time by inhibiting fibrinolysis by cleaving thrombin-activatable fibrinolysis inhibitor (TAFI, aka carboxypeptidase B2) into its active form. Thrombomodulin is a glycoprotein on the surface of endothelial cells that, in addition to binding thrombin, regulates C3b inactivation by factor I. Mutations in the thrombomodulin gene (THBD) have also been reported to be associated with atypical hemolytic-uremic syndrome (aHUS) .

• Description:

  Mouse Thrombomodulin ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Thbd in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Synonyms:

  Thrombomodulin; TM; Fetomodulin; CD141; Thbd

• Gene Name:

  Thrombomodulin

• UniProt:

  P15306

• Reactivity:

  Mouse

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: L17-S517

• Tissue Specificity:

  Endothelial cells are unique in synthesizing thrombomodulin.

• Applications:

  ELISA

• Field of Research:

  Cardiovascular, Stem Cells

• Sample Type:

  Cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

• Detection Range:

  62.5 pg/ml - 4,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Function:

  Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca) . Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.

• Storage Conditions:

  Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Other Gene Names:

  THBD

• Subcellular Location:

  Membrane; Single-pass type I membrane protein.

• Sequence Similarities:

  Contains 1 C-type lectin domain.

• Protein Name:

  Thrombomodulin