Mouse TGF-Beta 2 ELISA Kit PicoKine®

Mouse TGF-Beta 2 ELISA Kit PicoKine®

• Background: Transforming growth factor-beta 2 (TGF-beta 2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. This gene is mapped to 1q41. It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. TGF-beta 2 is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma (POAG). Studies have shown that TGF-beta 2 influences cultured trabecular meshwork cells, and it reduced outflow facility when perfused into cultured human anterior segments. In POAG, elevated expression of Gremlin by TM cells inhibited BMP4 antagonism of TGF-beta 2 and led to increased extracellular matrix deposition and elevated IOP.
• Description: Mouse TGF-Beta 2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tgfb2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: Transforming growth factor beta-2;TGF-beta-2;Latency-associated peptide;LAP;Tgfb2;
• CAS Number: 7732-18-5
• Gene Name: Transforming growth factor beta-2
• Gene ID: 21808
• UniProt: P27090
• Reactivity: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: A303-S414
• Applications: ELISA
• Standard: 10 ng/tube
• Sensitivity: <10 pg/ml
• Function: TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth.
• : heparin, EDTA or citrate
• Sample Type: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
• Detection Range: 31.2 pg/ml - 2,000 pg/ml
• Components: Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Calculated Molecular Weight: 47589 MW
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	98	90	99	91	94	4.03	4.2%
  Sample 2	235	221	227	238	230	6.68	2.9%
  Sample 3	827	847	843	932	862	40.95	4.7%

  *number of samples for each test n=16.
• Specificity: Natural and recombinant mouse TGF-beta 2
• Subcellular Location: Secreted.
• Protein Name: Transforming growth factor beta-2