Human ADAM12 / MCMP ELISA Kit PicoKine®

• Background:

  ADAM12 (Disintegrin and metalloproteinase domain-containing protein 12), also known as MLTN, is an enzyme that in humans is encoded by the ADAM12 gene. This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis. By RT-PCR and immunoblot analyses that expression of mouse Adam12 increases during muscle regeneration, while the levels of other ADAMs remain constant. Immunofluorescence analysis revealed staining of small, newly formed muscle fibers in regenerating but not normal adult muscle cells. By using of fluorescence in situ hybridization, the ADAM12 gene is mapped to human chromosome 10q26.3.

• Description:

  Human ADAM12 / MCMP ELISA Kit PicoKine® (96 Tests) . Quantitate Human ADAM12 in cell culture supernatants, serum, plasma (heparin) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Synonyms:

  Disintegrin and metalloproteinase domain-containing protein 12; ADAM 12;3.4.24.-; Meltrin-alpha; ADAM12; MLTN; UNQ346/PRO545

• Gene Name:

  Disintegrin and metalloproteinase domain-containing protein 12

• UniProt:

  O43184

• Reactivity:

  Human

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: CHO; Immunogen sequence: R29-S513

• Tissue Specificity:

  Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas.

• Applications:

  ELISA

• Field of Research:

  Cancer, Cell Adhesion, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Integrins, Invasion/Microenvironment, Metalloprotease, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction

• Sample Type:

  Cell culture supernatants, serum, plasma (heparin) and urine.

• Detection Range:

  156 pg/ml - 10,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Function:

  Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors (By similarity) . .

• Storage Conditions:

  Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Other Gene Names:

  ADAM12

• Subcellular Location:

  Isoform 1: Cell membrane; Single-pass type I membrane protein.

• Sequence Similarities:

  Contains 1 disintegrin domain.

• Protein Name:

  Disintegrin and metalloproteinase domain-containing protein 12