Human ADAMTS13 ELISA Kit PicoKine®

Human ADAMTS13 ELISA Kit PicoKine®

• Background: ADAMTS13 is a zinc-containing metalloprotease enzyme. The ADAMTS13 gene is mapped to chromosome 9q34 by genomic sequence analysis. It is reported that a metal-containing proteolytic enzyme (metalloprotease) in normal plasma cleaves the peptide bond between tyrosine at position 842 and methionine at position 843 in monomeric subunits of von Willebrand factor, thereby degrading the large multimers. And it is confirmed that the ADAMTS13 gene encodes the von Willebrand factor-cleaving protease (VWFCP). ADAMTS13 is secreted in blood and degrades large vWf multimers, decreasing their activity.
• Description: Human ADAMTS13 ELISA Kit PicoKine® (96 Tests). Quantitate Human ADAMTS13 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: A disintegrin and metalloproteinase with thrombospondin motifs 13;ADAM-TS 13;ADAM-TS13;ADAMTS-13;3.4.24.87;von Willebrand factor-cleaving protease;vWF-CP;vWF-cleaving protease;ADAMTS13;C9orf8;UNQ6102/PRO20085;
• CAS Number: 7732-18-5
• Gene Name: A disintegrin and metalloproteinase with thrombospondin motifs 13
• Gene ID: 11093
• UniProt: Q76LX8
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: CHO; Immunogen sequence: Q34-W688
• Applications: ELISA
• Sensitivity: <20 pg/ml
• Function: Cleaves the vWF multimers in plasma into smaller forms thereby controlling vWF-mediated platelet thrombus formation. .
• : heparin, EDTA or citrate
• Sample Type: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
• Detection Range: 0.78 ng/ml - 50 ng/ml
• Storage Conditions: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Calculated Molecular Weight: 153604 MW
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	1395	1323	1392	1567	1419	90.03	6.3%
  Sample 2	7236	8286	7812	8064	7849	391.89	4.9%
  Sample 3	20816	20291	22433	22964	21626	1104.51	5.1%

  *number of samples for each test n=16.
• Specificity: Natural and recombinant human ADAMTS13