Mouse TREM-1 ELISA Kit PicoKine®

Mouse TREM-1 ELISA Kit PicoKine®

• Background: Trem1, Triggering receptor expressed on myeloid cells-1, is encoded by Trem1 gene. The expression of Trem1 is in monocytes and neutrophils but not in lymphocytes, dendritic cells, or other cell types. Trem1 is a 30-kD glycoprotein that is reduced to 26 kD by deglycosylation, in agreement with the predicted molecular mass. The Trem1 gene which contains 4 exons maps to chromosome 6p21.1, within a TREM gene cluster and the mouse Trem1 gene maps to chromosome 17 in a region that shows homology of synteny to human chromosome 6. The expression of Trem1 is upregulated by stimulation with lipopolysaccharide (LPS), gram-negative bacteria, and fungi. Cross-linking of Trem1 on neutrophils induces interleukin-8 (IL8) and myeloperoxidase secretion, while cross-linking on monocytes induces not only secretion of IL8 but also of monocyte chemotactic protein-1 (MCP1, or SCYA2) and tumor necrosis factor (TNF); MCP1 and TNF secretion could be further upregulated by LPS-mediated priming. Trem1 engagement also induces upregulation of adhesion molecules (e.g., ITGB1) and costimulatory molecules (e.g., CD40). Trem1 is associated with DAP12 (TYROBP), a molecule frequently associated with activating receptors.
• Description: Mouse TREM-1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Trem1 in cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: Triggering receptor expressed on myeloid cells 1;TREM-1;CD354;Trem1;
• CAS Number: 7732-18-5
• Gene Name: Triggering receptor expressed on myeloid cells 1
• Gene ID: 58217
• UniProt: Q9JKE2
• Reactivity: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: A21-S202
• Applications: ELISA
• Sensitivity: <10 pg/ml
• Function: Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock (By similarity). .
• : heparin or EDTA
• Sample Type: cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates.
• Detection Range: 31.2 pg/ml - 2,000 pg/ml
• Storage Conditions: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Calculated Molecular Weight: 25409 MW
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	104	89	102	94	97	6.05	6.2%
  Sample 2	219	224	232	232	226	5.53	2.4%
  Sample 3	892	782	872	830	844	42.21	5%

  *number of samples for each test n=16.
• Specificity: Natural and recombinant mouse TREM-1