Mouse DAN/NBL1 ELISA Kit PicoKine®

Mouse DAN/NBL1 ELISA Kit PicoKine®

• Background: Differential screening-selected gene aberrative in neuroblastoma (DAN) is a member of the DAN family of secreted glycoproteins that are putative BMP antagonists. The NBL1 gene, also known as DAN, is originally cloned from a normal rat fibroblast cDNA library by a differential screening method. The human DAN gene is mapped to chromosome 1p36.13-p36. It is found that the DAN gene possesses a tumor suppressive activity when overexpressed in v-src transformed cells.
• Description: Mouse DAN/NBL1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Nbl1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: Neuroblastoma suppressor of tumorigenicity 1;N03;Zinc finger protein DAN;Nbl1;Dan, Dana;
• CAS Number: 7732-18-5
• Gene Name: Neuroblastoma suppressor of tumorigenicity 1
• Gene ID: 17965
• UniProt: Q61477
• Reactivity: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: A17-D178
• Applications: ELISA
• Sensitivity: <10 pg/ml
• Function: Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process.
• : heparin or EDTA
• Sample Type: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
• Detection Range: 62.5 pg/ml - 4,000 pg/ml
• Storage Conditions: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Calculated Molecular Weight: 19107 MW
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	84	77	79	90	82	5.02	6.1%
  Sample 2	591	589	657	668	626	36.46	5.8%
  Sample 3	1510	1598	1670	1616	1598	57.55	3.6%

  *number of samples for each test n=16.
• Specificity: Natural and recombinant mouse DAN