Mouse P-Selectin / CD62P ELISA Kit PicoKine®

• Background:

  P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is stored in secretory granules and expressed at the plasma membrane after cell activation. It is known to play an important role in atherosclerosis. The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1 (PSGL-1). The standard product used in this kit is recombinant mouse P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 42-709 amino acids sequence with the molecular mass of 99.1KDa. As a result of glycosylation, the molecular mass of 190-200KDa is revealed by SDS-PAGE.

• :

  citrate

• Description:

  Mouse P-Selectin / CD62P ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Selp in cell culture supernatants, serum and plasma (citrate). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	596	594	610	551	587	22.09	3.7%
  Sample 2	2016	2063	1888	1979	1986	64.18	3.2%
  Sample 3	7521	7765	7893	8387	7891	315.75	4%

  *number of samples for each test n=16.

• Synonyms:

  P-selectin;CD62 antigen-like family member P;Granule membrane protein 140;GMP-140;Leukocyte-endothelial cell adhesion molecule 3;LECAM3;Platelet activation dependent granule-external membrane protein;PADGEM;CD62P;Selp;Grmp;

• Gene Name:

  P-selectin

• Gene ID:

  20344

• UniProt:

  Q01102

• Reactivity:

  Mouse

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: W42-A709

• Tissue Specificity:

  Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

• Applications:

  ELISA

• Standard:

  20 ng/tube

• Sample Type:

  cell culture supernatants, serum and plasma (citrate).

• Detection Range:

  312 pg/ml - 20,000 pg/ml

• Sensitivity:

  <5 pg/ml

• Function:

  Ca (2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. .

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  83099 MW

• Specificity:

  Natural and recombinant mouse sP-Selectin

• Subcellular Location:

  Membrane; Single-pass type I membrane protein.

• Sequence Similarities:

  Belongs to the selectin/LECAM family.

• Protein Name:

  P-selectin

• CAS Number:

  7732-18-5