Mouse MMP-3/Stromelysin-1 ELISA Kit PicoKine®

Mouse MMP-3/Stromelysin-1 ELISA Kit PicoKine®

• Background: Matrix metalloproteinase-3 (MMP-3) also called stromelysin or transin, is a proteoglycanase closely related to collagenase (MMP1) with a wide range of substrate specificities. The complete primary structure for human MMP-3, which has 477 residues including a 17-residue signal peptide. MMP-3 and collagenase are 54% identical in sequence, suggesting a common origin for the evolution of the two proteinases. MMP-3 and collagenase expression are coordinately modulated in synovial fibroblast cultures. MMP-3 is a secreted metalloprotease produced predominantly by connective tissue cells. Together with other metalloproteases, it can synergistically degrade the major components of the extracellular matrix. It is capable of degrading proteoglycan, fibronectin, laminin, and type IV collagen, but not interstitial type I collagen. MMP-3 genotype may be an important determinant of vascular remodeling and age-related arterial stiffening, with the heterozygote having the optimal balance between matrix accumulation and deposition.
• Description: Mouse MMP-3 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Mmp3 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: Stromelysin-1;SL-1;3.4.24.17;EMS-2;Matrix metalloproteinase-3;MMP-3;Transin-1;Mmp3;
• CAS Number: 7732-18-5
• Gene Name: Stromelysin-1
• Gene ID: 17392
• UniProt: P28862
• Reactivity: Mouse
• Cross Reactivity: There is cross-reactivity with MMP-10 approximately 2% and no detectable cross-reactivity with other MMPs.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: Y18-C477
• Applications: ELISA
• Standard: 10 ng/tube
• Sensitivity: <10 pg/ml
• Function: Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
• : heparin
• Sample Type: cell culture supernatants, serum and plasma (heparin).
• Detection Range: 156 pg/ml - 10,000 pg/ml
• Components: Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Calculated Molecular Weight: 53845 MW
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	281	283	271	287	280	5.89	2.1%
  Sample 2	1427	1569	1617	1589	1550	73.31	4.7%
  Sample 3	4432	4951	4788	4684	4713	188.46	3.9%

  *number of samples for each test n=16.
• Specificity: Natural and recombinant mouse total MMP-3
• Subcellular Location: Secreted, extracellular space, extracellular matrix .
• Sequence Similarities: Belongs to the peptidase M10A family.
• Protein Name: Stromelysin-1