Mouse IL-3 ELISA Kit PicoKine®

Mouse IL-3 ELISA Kit PicoKine®

• Background: The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor (CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types. Interleukin-3 (IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3. The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide. The mouse IL 3 gene is located on chromosome 11. The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5. The standard product used in this kit is recombinant mouse IL-3, consisting of 135 amino acids with the molecular mass of 15KDa.
• Description: Mouse IL-3 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Il3 in cell culture supernatants and serum. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: Interleukin-3;IL-3;Hematopoietic growth factor;Mast cell growth factor;MCGF;Multipotential colony-stimulating factor;P-cell-stimulating factor;Il3;Csfmu, Il-3;
• CAS Number: 7732-18-5
• Gene Name: Interleukin-3
• Gene ID: 16187
• UniProt: P01586
• Reactivity: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: A27-C166
• Applications: ELISA
• Sample Type: cell culture supernatants and serum.
• Detection Range: 7.8 pg/ml - 500 pg/ml
• Sensitivity: <1 pg/ml
• Function: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.
• Storage Conditions: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Calculated Molecular Weight: 18540 MW
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	16	17	16	17	16	0.5	3.1%
  Sample 2	101	102	103	94	100	3.53	3.5%
  Sample 3	278	254	257	231	255	16.65	6.5%

  *number of samples for each test n=16.
• Specificity: Natural and recombinant mouse IL-3