Human GM-CSF ELISA Kit PicoKine®

• Background:

  Granulocyte-macrophage colony-stimulating factor (GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts ly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375.

• Description:

  Human GM-CSF ELISA Kit PicoKine® (96 Tests) . Quantitate Human CSF2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Synonyms:

  Granulocyte-macrophage colony-stimulating factor; GM-CSF; Colony-stimulating factor; CSF; Molgramostin; Sargramostim; CSF2; GMCSF

• Gene Name:

  Granulocyte-macrophage colony-stimulating factor

• UniProt:

  P04141

• Reactivity:

  Human

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: E.coli; Immunogen sequence: A18-E144

• Applications:

  ELISA

• Field of Research:

  Cytokines, Immunology, Innate Immunity

• Sample Type:

  Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

• Detection Range:

  15.6 pg/ml - 1,000 pg/ml

• Sensitivity:

  <1 pg/ml

• Function:

  Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

• Storage Conditions:

  Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Other Gene Names:

  CSF2

• Subcellular Location:

  Secreted.

• Sequence Similarities:

  Belongs to the GM-CSF family.

• Protein Name:

  Granulocyte-macrophage colony-stimulating factor