Mouse G-CSF / CSF3 ELISA Kit PicoKine®

• Background:

  Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of hormone-like glycoprotein that regulates hematopoietic cell proliferation and differentiation, and it almost exclusively stimulates the colony formation of granulocytes from committed precursor cells in semi-solid agar culture. G-CSF is also termed colony stimulating factor-3, and a single gene of which codes for a 177 or 180 amino acid mature protein of molecular weight 19,600. Functionally, it specifically stimulates the proliferation and differentiation of the progenitor cells for granulocytes. The effect of G-CSF on myeloid leukemias is unique among colony stimulating factors in driving the leukemic cells from a self-renewing malignant state to a mature differentiated phenotype with the concomitant loss of tumorigenicity. Besides, it also prevents cardiac remodeling after myocardial infarction by activating the Jak-Stat pathway in cardiomyocytes. The recombinant form of hG-CSF is capable of supporting neutrophil proliferation in a CFU-GM assay as well as early erythroid colonies and mixed colony formation. Human gene coding for G-CSF is assigned to the q21-q22 region of chromosome 17.3 The standard product used in this kit is recombinant Mouse G-csf, consisting of 179 amino acids with the molecular mass of 19KDa.

• Description:

  Mouse G-CSF / CSF3 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Csf3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 4pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Synonyms:

  Granulocyte colony-stimulating factor; G-CSF; Csf3; Csfg

• Gene Name:

  Granulocyte colony-stimulating factor

• UniProt:

  P09920

• Reactivity:

  Mouse

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: E.coli; Immunogen sequence: V31-A208

• Applications:

  ELISA

• Field of Research:

  Angiogenesis, Cancer, Cytokines, Immunology, Innate Immunity, Invasion/Microenvironment

• Sample Type:

  Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

• Detection Range:

  31.2 pg/ml - 2,000 pg/ml

• Sensitivity:

  <4 pg/ml

• Function:

  Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

• Storage Conditions:

  Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Other Gene Names:

  CSF3

• Subcellular Location:

  Secreted.

• Protein Name:

  Granulocyte colony-stimulating factor