Mouse FAS/Cd95 ELISA Kit PicoKine®

Mouse FAS/Cd95 ELISA Kit PicoKine®

• Background: Fas, also known as APO-1, CD95 and TNFRSF6, is a member of the nerve growth factor (NGF)/tumour necrosis factor (TNF) receptor superfamily and mediates apoptosis. The nucleotide sequence of the cDNAs reveales that the molecule coding for the Fas antigen determinant is a 319 amino acid polypeptide with a single transmembrane domain. The extracellular domain is rich in cysteine residue, and shows a similarity to that of human tumor necrosis factor receptors, human nerve growth factor receptor, and human B cell antigen CD40. The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 is previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. The APO-1 antigen is found to be a membrane glycoprotein of 48-kDa. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. Fas can play a role as an inducer of both neurite growth in vitro and accelerates recovery after nerve injury in vivo.
• Description: Mouse FAS/Cd95 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Fas in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 3pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: Tumor necrosis factor receptor superfamily member 6 ;Fas ;
• CAS Number: 7732-18-5
• Gene Name: Fas cell surface death receptor
• Gene ID: 14102
• UniProt: Q8C350
• Reactivity: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: Q22-R169
• Applications: ELISA
• Storage Conditions: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• : heparin, EDTA or citrate
• Sample Type: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
• Detection Range: 31.2 pg/ml - 2,000 pg/ml
• Sensitivity: <3 pg/ml
• Calculated Molecular Weight: 7116 MW
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	67	72	66	74	69	3.34	4.8%
  Sample 2	227	219	205	237	222	11.7	5.2%
  Sample 3	869	913	935	934	912	26.74	2.9%

  *number of samples for each test n=16.
• Specificity: Natural and recombinant mouse FAS