Anti-Macrophage Inflammatory Protein 1 beta/CCL4 Antibody Picoband®

Anti-Macrophage Inflammatory Protein 1 beta/CCL4 Antibody Picoband®

• Background: Chemokine (C-C motif) ligand 4, also known as CCL4, is a protein which in human is encoded by the CCL4 gene. By analysis of somatic cell hybrids and by in situ hybridization, the CCL4 gene was assigned to 17q21-q23. It is a CC chemokine with specificity for CCR5 receptors. And it is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize CCL4. Based on functional analysis of CCL4, one or more additional beta chemokine receptors that transduce CCL4 signals must also exist. MIP-1alpha and CCL4 have been shown to lack affinity for the erythrocyte surface chemokine receptor/Duffy antigen that binds many alpha as well as beta chemokines (11, 14).
• Description: Boster Bio Anti-Macrophage Inflammatory Protein 1 beta/CCL4 Antibody Picoband® catalog # PB9572. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: C-C motif chemokine 4;G-26 T-lymphocyte-secreted protein;HC21;Lymphocyte activation gene 1 protein;LAG-1;MIP-1-beta (1-69);Macrophage inflammatory protein 1-beta;MIP-1-beta;PAT 744;Protein H400;SIS-gamma;Small-inducible cytokine A4;T-cell activation protein 2;ACT-2;MIP-1-beta (3-69);CCL4;LAG1, MIP1B, SCYA4;
• CAS Number: 9007-83-4
• Gene Name: C-C motif chemokine 4
• Gene ID: 6351
• UniProt: P13236
• Host: Rabbit
• Reactivity: Human
• Cross Reactivity: No cross-reactivity with other proteins
• Immunogen: E. coli-derived human CCL4 recombinant protein (Position: A24-N92). Human CCL4 shares 76.8% and 79.7% amino acid (aa) sequence identity with mouse and rat CCL4, respectively.
• Clonality: Polyclonal
• Applications: ELISA,WB
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Function: Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta (3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5- mediated entry of HIV-1 in T-cells. MIP-1-beta (3-69) is also a ligand for CCR1 and CCR2 isoform B. .
• References & Citations: 1. Bystry RS, Aluvihare V, Welch KA, Kallikourdis M, Betz AG (December 2001). "B cells and professional APCs recruit regulatory T cells via CCL4". Nat. Immunol. 2 (12): 1126–32. 2. Cocchi F, DeVico AL, Garzino-Demo A, Arya SK, Gallo RC, Lusso P (December 1995). Science (journal) 270 (5243): 1811–5. 3. Irving SG, Zipfel PF, Balke J, McBride OW, Morton CC, Burd PR, Siebenlist U, Kelly K (June 1990). Nucleic Acids Res. 18 (11): 3261–70. 4. Kamin-Lewis R, Abdelwahab SF, Trang C, Baker A, DeVico AL, Gallo RC, Lewis GK (July 2001).
• Storage Conditions: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 10212 MW
• Observed Molecular Weight: 11 kDa
• Fragment: Rabbit IgG
• Specificity: No cross reactivity with other proteins.
• Applications Notes: ELISA, 0.1-0.5μg/ml, -
  Western blot, 0.1-0.5μg/ml, Human