Anti-c-Myc Antibody (Monoclonal, 9E10)

• Background:

  C-Myc is an oncogene that functions both in the stimulation of cell proliferation and in apoptosis. c-Myc elicits its oncogenic activity by causing immortalization, and to a lesser extent the transformation of cells, in addition to several other mechanisms. The c-MYC proto-oncogene encodes a transcription factor that is critical for cell growth and proliferation. It is one of the genes frequently altered in cancer cells in which it exhibits constitutive activity. Downregulation of c-Myc is critical for 2-Methoxyestradiol (2ME2) -induced oxidative stress and apoptosis in AML cells. And its up-regulation is important for promoting lymphocyte cell division, and demonstrating that GFP-c-Myc expression is a marker of proliferating lymphocytes in vivo.

• Description:

  Boster Bio Anti-c-Myc Antibody (Monoclonal, 9E10) catalog # MA1028. Tested in IHC, ICC, WB applications. This antibody reacts with Human.

• Synonyms:

  Myc proto-oncogene protein; Proto-oncogene c-Myc; Transcription factor p64; Myc

• Gene Name:

  Myc proto-oncogene protein

• UniProt:

  P09416

• Host:

  Mouse

• Reactivity:

  Human

• Cross Reactivity:

  No cross-reactivity with other proteins

• Immunogen:

  Synthetic peptide corresponding to residues 408-439 of the human p62c-Myc protein.

• Clonality:

  Monoclonal

• Clone:

  Clone: Clone: 9E10

• Tissue Specificity:

  Expressed on the plasma membrane of hepatocytes and in the narrow apical portions of supporting cells in the vomeronasal sensory epithelium. Detected in the type III alveolar cells of the lung, in the proliferative crypt epithelium of the small intestine and in the older intragemmal cells of the tongue. .

• Applications:

  IHC, ICC, WB

• Field of Research:

  Cancer, Cell Cycle, Cell Differentiation, Domain Families, Epigenetics and Nuclear Signaling, Hlh/Leucine Zipper, Nuclear, Oncoproteins, Oncoproteins/Suppressors, Signaling Pathways, Stem Cells, TGF Beta, Transcription, Transcription Factors, Tumor Biomarkers

• Purification:

  Ascites

• Concentration:

  Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

• Function:

  Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'- CAC[GA]TG-3'. Activates the transcription of growth-related genes. .

• Storage Conditions:

  Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

• Fragment:

  Mouse IgG1

• Applications Notes:

  Immunohistochemistry (Paraffin-embedded Section), 5μg/ml, Human, By Heat
  Immunocytochemistry , 1μg/ml, Human, -
  Western blot, 1μg/ml, Human
  

• Other Gene Names:

  MYC

• Subcellular Location:

  Nucleus, nucleoplasm . Nucleus, nucleolus .

• Sequence Similarities:

  Contains 1 bHLH (basic helix-loop-helix) domain.

• Protein Name:

  Myc proto-oncogene protein