Human Tryptase/TPSAB1,B2 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Human Tryptase/TPSAB1,B2 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

• Background: Tryptases are serine proteases implicated in asthma and are highly expressed in human mast cells. They are derived from at least 4 nonallelic genes clustered on chromosome 16p13.3: TPSAB1, which represents the alpha and beta-I tryptase alleles; TPSB2, which represents the beta-II and beta-III tryptase alleles; TPSG1; and TPSD1. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis. Tryptase is less likely to be elevated in food allergy reactions as opposed to other causes of anaphylaxis.
• Description: Human Tryptase/TPSAB1,B2 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human Tryptase/TPSAB1,B2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
• Synonyms: Tryptase beta-2;Tryptase-2;3.4.21.59;Tryptase II;TPSB2;TPS2;
• CAS Number: 7732-18-5
• Gene Name: Tryptase/TPSAB1,B2
• Gene ID: 7177
• UniProt: Q15661
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: M1-V275
• Applications: ELISA
• Sample Type: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
• Function: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. .
• : heparin or EDTA
• Detection Range: 156 pg/ml - 10,000 pg/ml
• Storage Conditions: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Calculated Molecular Weight: 30515 MW
• Specificity: Natural and recombinant human Tryptase/TPSAB1,B2