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SPI1 Antibody

CAT: 0013-GTR18126033Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18126033Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
SPI1 Antibody
Product Name Alternative
PU.1, SPI1, spleen focus forming virus (SFFV) proviral integration oncogene spi1 , hCG_25181, SFPI1, SPI-1, SPI-A, 31 kDa transforming protein, SPI-1 proto-oncogene, hematopoietic transcription factor PU.1
UniProt
P17947
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-DLYQRQTHEYY
Target
SPI1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 40kDa band observed in lysates of DAUDI and K562 (calculated MW of 31.2kDa according to NP_001074016.1 and of 31.1kDa according to NP_003111.2) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Harendza S et al, J Biol Chem. 2000 Jun 30;275 (26) :19552-9.; PMID: 10867017) . Recommended concentration: 0.03-0.1μg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen and Colon (lymphoid aggregate) . Recommended concentration: 3.75μg/mL.
Prediction Reactivity
Rat
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_001074016.1, NP_003111.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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