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TRIM55 Antibody

CAT: 0013-GTR18124404Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18124404Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
TRIM55 Antibody
Product Name Alternative
TRIM55, RNF29, MURF-2, ring finger protein 29, for muscle specific ring finger 2, tripartite motif-containing 55, muscle specific ring finger 2
UniProt
Q9BYV6
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-HIFSFSWLNSLNE
Target
TRIM55
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Musculoskeletal & Connective Tissue Research
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 58kDa band observed in lysates of cell line HeLa (calculated MW of 60.5kDa according to NP_908973.1) . Recommended concentration: 1-3μg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Pig, Rat and Mouse Heart, and Pig and Mouse Skeletal Muscle for this particular batch.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 10μg/mL.Immunofluorescence: Strong expression of the protein seen in the Golgi and cytoplasm of U2OS cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10μg/mL.
Prediction Reactivity
Canine, Mouse, Porcine, Rat
Tested Applications
ELISA, FC, IF, IHC, WB
NCBI Accession Number
NP_908973.1, NP_908974.1, NP_908975.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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