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PCK1 Antibody

CAT: 0013-GTR18124293Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18124293Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
PCK1 Antibody
Product Name Alternative
PCK1, phosphoenolpyruvate carboxykinase 1 (soluble), HGNC:8724, MGC22652, PEPCK1, PEPCKC, PEP carboxykinase, cytosolic phosphoenolpyruvate carboxykinase 1, phosphoenolpyruvate carboxylase, phosphopyruvate carboxylase, PEPCK-C
UniProt
P35558
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-HVNWFRKDKEGK
Target
PCK1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave bands at approx 70kDa, 45kDa and 28kDa in Human Adipose, Kidney and Liver lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the additional bands we observe given the calculated size of 69.2 kDa according to NP_002582.2. All three detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) .Immunohistochemistry:Paraffin embedded Human Cerebral Cortex, Kidney and Liver. Recommended concentration: 2μg/mL.
Prediction Reactivity
Bovine, Canine, Mouse, Porcine, Rat
Tested Applications
ELISA, IHC
NCBI Accession Number
NP_002582.3
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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