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UPF3A Antibody

CAT: 0013-GTR18126990Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18126990Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
UPF3A Antibody
Product Name Alternative
UPF3A, UPF3 regulator of nonsense transcripts homolog A (yeast), HUPF3A, RENT3A, UPF3, OTTHUMP00000018789, UPF3 regulator of nonsense transcripts homolog A
UniProt
Q9H1J1
Immunogen
The immunogen for this antibody is: C-KETDKQKKIAEKE
Target
UPF3A
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Signal Transduction
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave an approx 48kDa band in lysates of cell lines HeLa and Kelly after 0.3μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 51kDa according to NP_542418.1 and the reported sizes of 65 to 70kDa from different sources. The 48kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Prediction Reactivity
Bovine, Human
Tested Applications
ELISA
NCBI Accession Number
NP_542418.1, NP_075387.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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