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VPS29 Antibody

CAT: 0013-GTR18123934Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18123934Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
VPS29 Antibody
Product Name Alternative
VPS29, vacuolar protein sorting 29 (yeast), DC15, DC7, DKFZp564F0223, FLJ20492, PEP11 , retromer protein, vacuolar protein sorting 29, vacuolar protein sorting 29 (yeast homolog), vacuolar sorting protein VPS29/PEP11, x 007 protein
UniProt
Q9UBQ0
Reactivity
Human
Immunogen
The immunogen for this antibody is: CTGNLCTKESYDYLK
Target
VPS29
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 60kDa band in Human Spleen lysates after 0.3μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 20.5kDa according to NP_057310.1 and 20.9kDa according to NP_476528.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Uterus shows vesicular and membranous staining in the endometrial glands. Recommended concentration, 4-8μg/mL.
Prediction Reactivity
Canine, Mouse, Rat
Tested Applications
ELISA, IHC
NCBI Accession Number
NP_057310.1, NP_476528.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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