Chaps Lysis and Immunoprecipitation Buffer

• Description:

  CHAPS Buffer Strong cell lysis and protein extraction capacity while preserving protein conformation and protein-protein interaction for co-immunoprecipitation (Co-IP) analysis. Can be used with tissues (see product citation 1 below) as well as culture cells. Effective as a stand alone reagent for cell lysis, protein extraction and subsequent Western blot in addition to immunoprecipitation. Contains a non-amine buffer (HEPES) enabling cross-linking with NHS-ester derivatives. Ingredients:  0.5% CHAPS, HEPES, NaClCompatible with Bradford, Lowry and BCA protein assays. 60 mL volume:  Suitable for two hundred 10 cm culture plates, or six hundred wells in 6-well dishes.  120 mL volume for four hundred 10 cm culture plates or 1200 wells in 6-well dishes.

• pH:

  pH 7.4

• Storage Conditions:

  4°C