QuicKey Pro Human PLAP/ALPP (Placental Alkaline Phosphatase) ELISA Kit

QuicKey Pro Human PLAP/ALPP (Placental Alkaline Phosphatase) ELISA Kit

• Abbreviation: PLAP
• Synonyms: ALP; ALPP; Alkaline phosphatase; PALP; PLAP; PLAP-1; Placental
• CAS Number: 7732-18-5
• UniProt: P05187
• Cross Reactivity: This kit recognizes PLAP in samples.No significant cross-reactivity or interference between PLAP and analogues was observed.
• Target: Human
• Type: Sandwich-ELISA
• Applications: ELISA
• Field of Research: Cancer; Tags and Cell Markers
• Detection Method: Colorimetric method; ELISA; Sandwich
• Assay Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PLAP/ALPP. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human PLAP/ALPP are added to the micro ELISA plate wells. Human PLAP/ALPP in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human PLAP/ALPP.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PLAP/ALPP. The concentration of Human PLAP/ALPP in the samples is then determined by comparing the OD of the samples to the standard curve.
• Assay Performance Time: 1 h 30 min
• Sample Type: Serum, plasma and other biological fluids
• Detection Range: 78.13-5000 pg/mL
• Precision: Both intra-CV and inter-CV are < 10%
• Recovery: 80%-120%
• Shipping Conditions: Ice bag
• Storage Conditions: 2-8°C, 6 months
• Applications Notes: This ELISA kit applies to the in vitro quantitative determination of Human PLAP/ALPP concentrations in serum, plasma and other biological fluids.