SingleFlowEx CD45R0 PerCP-Cy1535.5

SingleFlowEx CD45R0 PerCP-Cy1535.5

• Description: SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples. SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.
• Certification: RUO, GMP
• Clone: UCHL1
• Validated Applications: FC
• Concentration: 0.030 mg/mL
• Format: PerCP-Cy1535.5
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Components: 1 vial of monoclonal antibody reagent. The content (1 mL) is sufficient for min. 100 Tests.
• References & Citations: *Norton AJ, Ramsay AD, Smith SH, Beverley PC, Isaacson PG: Monoclonal antibody (UCHL1) that recognises normal and neoplastic T cells in routinely fixed tissues. J Clin Pathol. 1986 Apr39(4):399-405. , URL: https://www.ncbi.nlm.nih.gov/pubmed/2422219?dopt=A,*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995)., URL: ,*Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989)., URL: ,*Merkenschlager M, Terry L, Edwards R, Beverley PC: Limiting dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T cell memory formation. Eur J Immunol. 1988 Nov18(11):1653-61., URL: https://www.ncbi.nlm.nih.gov/pubmed/2974420?dopt=A,*Beverley PC, Merkenschlager M, Terry L: Phenotypic diversity of the CD45 antigen and its relationship to function. Immunol Suppl. 19881:3-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/2968306?dopt=A,*Terry LA, Brown MH, Beverley PC: The monoclonal antibody, UCHL1, recognizes a 180,000 MW component of the human leucocyte-common antigen, CD45. Immunology. 1988 Jun64(2):331-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/2455685?dopt=A,*Akbar AN, Terry L, Timms A, Beverley PC, Janossy G: Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T cells. J Immunol. 1988 Apr 1140(7):2171-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/2965180?dopt=A,*Leukocyte Typing III., McMichael A. J. et al (Eds.), Oxford University Press (1987)., URL: ,*Beverley PC.: Human T cell subsets. Immunol Lett. 1987 Apr14(4):263-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/2953677?dopt=A,*Smith SH, Brown MH, Rowe D, Callard RE, Beverley PC: Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1. Immunology. 1986 May58(1):63-70., URL: https://www.ncbi.nlm.nih.gov/pubmed/2423439?dopt=A,*Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug20(8):1174-1178. doi: 10.1038/s41592-023-01846-7., URL: https://pubmed.ncbi.nlm.nih.gov/37468619/,*Use of this product has not been published., URL: ,
• Other References: *Li FJ, Tsuyama N, Ishikawa H, Obata M, Abroun S, Liu S, Otsuyama K, Zheng X, Ma Z, Maki Y, Kawano MM: A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma. Blood. 2005 Apr 15105(8):3295-302., URL: https://www.ncbi.nlm.nih.gov/pubmed/15626731?ordin,*Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis. Brain Pathol. 2006 Oct16(4):256-65., URL: https://www.ncbi.nlm.nih.gov/pubmed/17107594?ordin,*Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ. Combinations of CD45 isoforms are crucial for immune function and disease. J Immunol. 2006 Mar 15176(6):3417-25., URL: https://www.ncbi.nlm.nih.gov/pubmed/16517710?ordin,*Bijian K, Zhang L, Shen SH: Collagen-mediated survival signaling is modulated by CD45 in Jurkat T cells. Mol Immunol. 2007 Jul44(15):3682-90., URL: https://www.ncbi.nlm.nih.gov/pubmed/17524482?ordin,*Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2007 Dec 19 , URL: https://www.ncbi.nlm.nih.gov/pubmed/18157742?ordin,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.