Anti-Myelin Basic Protein Antibody

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CAT:

14-24201-1

Size:

500 µg

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Anti-Myelin Basic Protein Antibody

Description: Mouse Monoclonal Antibody specific to Myelin Basic Protein
Alternative Name: MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein
Gene Name: MBP
NCBI Gene ID: MBP
UniProt: P02686
Cellular Locus: Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic side of myelin.; [Isoform 3]: Nucleus
Host: Mouse
Reactivity: Human, Mouse, Rat, Rabbit, Sheep, Goat, Primate
Immunogen: Purified human myelin basic protein.
Target Antigen: Myelin basic protein
Target: Myelin Basic Protein
Clonality: Monoclonal
Isotype: IgG2b
Type: Antibody
Applications: WB, IHC, ELISA
Field of Research: Neuroscience
Purification Method: Purified by Protein G affinity chromatography
Concentration: Lot Specific
Dilution: Dilute in PBS or medium that is identical to that used in the assay system.
Format: Purified
Form: Liquid
Buffer: Phosphate Buffered Saline
Function: The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. {PubMed:8544862}.
Additionnal Information: This antibody may be used in ELISA, immunohistochemistry, and Western blot procedures for detection and/or quantitation of myelin basic protein.ELISA: Human myelin basic protein coated on ELISA wells at 5ug/ml; binding detected with HRP-conjugated goat anti-mouse IgG + ABTS substrate.
Storage Conditions: This antibody is stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Specificity: This antibody reacts with residues 130-136 in human myelin basic protein (21.5kDa and 18.5kDa molecular forms). It also recognizes primate, rabbit, sheep, goat, rat, and mouse myelin basic protein.
Formulation: PBS, pH 7.4.
Buffer pH: pH 7.4

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