CD63 Mouse Monoclonal Antibody

CAT: 0013-GTR18111613-01Size: 30 µLDry Ice: NoHazardous: No

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CAT#:0013-GTR18111613-01Size:30 µL

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

The CD63 Antibody is suitable for FC, IF. It is a Monoclonal, Unconjugated antibody which raised against Native purified human CD63. Purification: The antibody was purified by affinity chromatography.

Product Name Alternative

MLA1; TSPAN30; CD63 antigen; Granulophysin; Lysosomal-associated membrane protein 3; LAMP-3; Melanoma-associated antigen ME491; OMA81H; Ocular melanoma-associated antigen; Tetraspanin-30; Tspan-30; CD antigen CD63

UniProt

P08962

Reactivity

Human

Immunogen

Native purified human CD63.

Target

CD63

Clonality

Monoclonal

Clone

BJ7E8

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

The antibody was purified by affinity chromatography.

Dilution

IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured) .

Tested Applications

FC, IF

Host or Source

Mouse

Preservative

PBS, pH 7.3, and 0.02% sodium azide.

Isotype

Mouse IgG1

Entrez

967

UniProtKB · P08962

CD63 antigen

CD63_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P08962
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: CD63
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: —
Secondary accessions: F8VZE2, Q5TZP3, Q8N6Z9, Q9UCG6

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneEndosomeLysosomeMembraneSecreted

PTM

GlycoproteinLipoproteinPalmitate

Biological process

Protein transportTransport

Domain

TransmembraneTransmembrane helix

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