Mouse CD27 Ligand / CD70 Protein, Mouse IgG2a Fc Tag, low endotoxin

Mouse CD27 Ligand / CD70 Protein, Mouse IgG2a Fc Tag, low endotoxin

• Background: Cluster of Differentiation 70 (CD70) is also known as CD27 ligand (CD27L / CD27LG), TNFSF7, TNFSF7G, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and is a surface antigen found on activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor CD27 induces in priming, effector functions, differentiation and memory formation of T-cells, and thus is invloved in the biological processes including T-cell activation, the proliferation of costimulated T-cells, as well as the generation of cytolytic T-cells. CD70 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation. CD70 on mouse B cell has been shown to inhibit terminal differentiation of activated B cells into plasma cells and enhances commitment to memory B cell responses. CD70 induces proliferation and IFNγ production, on NK cells.
• Description: Mouse CD27 Ligand Protein, Mouse IgG2a Fc Tag (CDL-M5251) is expressed from human 293 cells (HEK293). It contains AA Gln 47 - Pro 195 (Accession # O55237-1).
• Specifications: This protein carries a mouse IgG2a Fc tag at the N-terminus. The protein has a calculated MW of 43.5 kDa. The protein migrates as 47-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Gln 47 - Pro 195
• Host: HEK 293
• Target: CD27 Ligand
• Conjugation: Unconjugated
• Tag: N-mFc(mIgG2a)
• Source: Mouse
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 0.1 EU per μg
• Purity: 95%
• Bioactivity: Immobilized Human CD27, His Tag (Cat. No. CD7-H522b) at 1 μg/mL (100 μL/well) can bind Mouse CD27 Ligand Protein, Mouse IgG2a Fc Tag (Cat. No. CDL-M5251) with a linear range of 0.3-5 ng/mL (QC tested).
• Format: Powder
• Buffer: 50 mM Tris, 100 mM Glycine, pH7.5
• Additives: Trehalose
• Molecular Weight: 43.5 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C