Recombinant Mouse LIF

• Product Name Alternative:

  CDF, Cholinergic Differentiation Factor, D factor, DIA, Differentiation inducing factor, differentiation inhibitory activity, Differentiation stimulating factor

• UniProt:

  P09056

• Expression Region:

  24-203

• Host:

  E.Coli

• Tag:

  N-terminal His Tag

• Applications:

  Western Blot, ELISA

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Measured by its ability to induce IL-6 secretion by M1 mouse myeloid leukemia cells. The ED50 for this effect is 0.2-0.6 ng/mL.

• Form:

  Lyophilized powder

• Reconstitution:

  Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.

• Molecular Weight:

  19.7 kDa

• Shipping Conditions:

  4°C with ice bag

• Storage Conditions:

  The lyophilized protein is stable at -20 °C for up to 1 year. For extended storage, it is recommended to further dilute in working aliquots after reconstitution. The protein solution is stable at ≤ -20 °C for 3 months, or 2-7 days at 2-8 °C under sterile conditions.Avoid repeated freeze/thaw cycle.

• Formulation:

  Lyophilized from a 0.2 μm filtered solution of 10 mM Hepes, 150 mM NaCl with 5% trehalose, pH 7.4.

• Organism Species:

  Mouse