Mouse IgG1 Negative Control

Mouse IgG1 Negative Control

• Background: This monoclonal antibody to IgG1 is a negative control for Immunofluorescence staining with Pure, FITC, Biotin, PE and APC mouse monoclonal antibodies of the IgG1 subclass. It is used to monitor the level of non-specific binding of mouse monoclonal antibodies to Human cell surface antigens.
• Synonyms: IgG1
• UniProt: P07750
• Host: Mouse
• Isotype: IgG1
• Clone: ZX3
• Conjugation: Unconjugated
• Type: Second Step Reagents
• Applications: Flow Cytometry
• Field of Research: Flow Cytometry
• Purification Method: Protein A/G Chromatography
• Assay Principle: Consult the appropriate fact sheet to determine the amount of antibodies to be used as a control for PBMC or Whole Blood. PBMC: Add 10 µL of MAB/10^6 PBMC in100 µL PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze. WHOLE BLOOD: Add10 µL of MAB/100 µL of Whole Blood. Mix gently and incubate for 15 minutes at room temperature 20ºC. Lyse the whole blood. Wash once with PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjµgates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at ?633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5 +: Excites at 488nm and emits at 670nm. Store protected from light. Optimal concentration should be evaluated by serial dilutions.
• Stability: See expiration date on vial
• Concentration: See vial for Concentration
• Form: Provided as solution in phosphate buffered saline with 0,08% sodium azide
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
• References & Citations: 1. Immunofluorescence Measurement in a Flow Cytometer using Low-Power Helium Neon Laser Excitation. Shapiro, H.M, Glazer, A.N., Christenson, L., Williams, J.M., and Strom, T. B. Cytometry 4,276, 1983. _x000D_ 2. Comparison of Helium Neon and Dye lasers for Excitation of Allophycocyanin. Loken, M.R., Kiej, J.F. and Kelly, K.,A. Cytometry 8, 96, 1987. Cy-5 + Portions of this product is manufactured under license from Carnegie Mellon University, U.S. Patent Number 5,268,486.
• Shipping Conditions: Ambient Temperature
• Storage Conditions: Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
• Functional Analysis: Flow Cytometry Staining