Indole-3-Butyric Acid (IBA)

Indole-3-Butyric Acid (IBA)

• Description: Indole-3-Butyric Acid (IBA) Powder is a plant growth regulator widely used to promote root growth in plant tissue culture applications.
• Synonyms: IBA; 4-[3-Indolyl]butyric Acid
• CAS Number: 133-32-4
• HS Code: 3808.93
• Applications: Stimulates adventitious root development and callus formation. IBA can be included in media formulations for micropropagation to enhance plantlet root development. IBA can be applied to the graft union to promote faster callus formation and successful graft healing. IBA can promote germination in certain seeds with dormancy requirements.
• Assay Protocol: Follow the given steps to prepare the tissue culture media using PCT’s IBA powder: Prepare liquid tissue culture media using PCT’s MS, DKW media, or any other alternative media based on your plant. Add sugar based on the quantity of media you’re making (generally, 30 grams of sugar is added for 1-liter media), 1 ml/L PPM, and 3-4 grams per liter of gellan gum or 6-8 grams per liter of agar. Then, add the desired quantity of IBA from the prepared IBA stock solution. Autoclave the medium for 20 minutes at 15 psi and 121°C. Your media is ready!
• Solubility: Soluble in NaOH or KOH
• Molecular Formula: C12H13NO2
• Features & Benefits: Easy to use. Used to supplement tissue culture media, such as MS media. Stimulates adventitious root development and callus formation. It is often used with other hormones to promote optimal plant growth and development.
• Storage Temperature: 2-8°C
• Shelf Life: 3 years from the manufacturing date
• Appearance: Cream to Tan Powder