SingleFlowEx CD11c APC-Cy1537

SingleFlowEx CD11c APC-Cy1537

• Description: SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples. SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.
• Certification: RUO, GMP
• Clone: BU15
• Validated Applications: FC
• Concentration: 0.020 mg/mL
• Format: APC-Cy1537
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Components: 1 vial of monoclonal antibody reagent. The content (1 mL) is sufficient for min. 100 Tests.
• References & Citations: *Van der Vieren M, Le Trong H, Wood CL, Moore PF, St John T, Staunton DE, Gallatin WM: A novel leukointegrin, alpha d beta 2, binds preferentially to ICAM-3. Immunity. 1995 Dec3(6):683-90., URL: http://www.ncbi.nlm.nih.gov/pubmed/8777714?ordinal,*Myou S, Zhu X, Boetticher E, Qin Y, Myo S, Meliton A, Lambertino A, Munoz NM, Hamann KJ, Leff AR: Regulation of adhesion of AML14.3D10 cells by surface clustering of beta2-integrin caused by ERK-independent activation of cPLA2. Immunology. 2002 Sep107(1):77-85., URL: http://www.ncbi.nlm.nih.gov/pubmed/12225365?ordina,*Sadhu C, Hendrickson L, Dick KO, Potter TG, Staunton DE: Novel Tools for Functional Analysis of CD11c: Activation-Specific, Activation-Independent, and Activating Antibodies. J Immunoassay Immunochem. 200829(1):42-57., URL: http://www.ncbi.nlm.nih.gov/pubmed/18080879?ordina,*Angel CE, Lala A, Chen CJ, Edgar SG, Ostrovsky LL, Dunbar PR: CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts. Int Immunol. 2007 Nov19(11):1271-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/17804688?itool=,*Rezzonico R, Imbert V, Chicheportiche R, Dayer JM: Ligation of CD11b and CD11c beta(2) integrins by antibodies or soluble CD23 induces macrophage inflammatory protein 1alpha (MIP-1alpha) and MIP-1beta production in primary human monocytes through a pathway dependent on nuclear factor-kappaB. Blood. 2001 May 1597(10):2932-40., URL: http://www.ncbi.nlm.nih.gov/pubmed/11342414?ordina,*Leukocyte Typing III., McMichael A. J. et al (Eds.), Oxford University Press (1987)., URL: ,*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995)., URL: ,*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997)., URL: ,*Hogg N, Takacs L, Palmer DG, Selvendran Y, Allen C: The p150,95 molecule is a marker of human mononuclear phagocytes: comparison with expression of class II molecules. Eur J Immunol. 1986 Mar16(3):240-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/3456894?ordinal,*Trowald-Wigh G, Johannisson A, H229kansson L: Canine neutrophil adhesion proteins and Fc-receptors in healthy dogs and dogs with adhesion protein deficiency, as studied by flow cytometry. Vet Immunol Immunopathol. 1993 Oct38(3-4):297-310., URL: http://www.ncbi.nlm.nih.gov/pubmed/8291207?ordinal,*Use of this product has not been published., URL: ,
• Other References: *Vorup-Jensen T, Chi L, Gjelstrup LC, Jensen UB, Jewett CA, Xie C, Shimaoka M, Linhardt RJ, Springer TA: Binding between the integrin alphaXbeta2 (CD11c/CD18) and heparin. J Biol Chem. 2007 Oct 19282(42):30869-77., URL: http://www.ncbi.nlm.nih.gov/pubmed/17699512?ordina,*Gang J, Choi J, Lee JH, Nham SU: Identification of critical residues for plasminogen binding by the alphaXI-domain of the beta2 integrin, alphaXbeta2. Mol Cells. 2007 Oct 3124(2):240-6., URL: http://www.ncbi.nlm.nih.gov/pubmed/17978577?ordina,*Sadhu C, Ting HJ, Lipsky B, Hensley K, Garcia-Martinez LF, Simon SI, Staunton DE: CD11c/CD18: novel ligands and a role in delayed-type hypersensitivity. J Leukoc Biol. 2007 Jun81(6):1395-403., URL: http://www.ncbi.nlm.nih.gov/pubmed/17389580?ordina,*Bullard DC, Hu X, Adams JE, Schoeb TR, Barnum SR: p150/95 (CD11c/CD18) expression is required for the development of experimental autoimmune encephalomyelitis. Am J Pathol. 2007 Jun170(6):2001-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/17525267?ordina,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.