Influenza B [Phuket/3073/2013 (B/Yamagata lineage) ] Hemagglutinin (HA) Protein, His Tag

• Background:

  Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Hemagglutinin also plays a major role in the determination of host range restriction and virulence. As a class I viral fusion protein, hemagglutinin is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane.

• Description:

  Influenza B [Phuket/3073/2013 (B/Yamagata lineage) ] HA Protein, His Tag (HAE-V52H4) is expressed from human 293 cells (HEK293) . It contains AA Asp 16- Ser 558 (Accession # EPI529345, GISAID) .

• Specifications:

  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 64.1 kDa. The protein migrates as 80-100 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  EPI529345

• Expression Region:

  Asp 16- Ser 558

• Host:

  HEK293

• Target:

  Hemagglutinin/HA (Influenza Virus)

• Conjugation:

  Unconjugated

• Tag:

  C-10xHis

• Source:

  Influenza

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized Influenza B [Phuket/3073/2013 (B/Yamagata lineage)] HA Protein, His Tag (Cat. No. HAE-V52H4) at 1 μg/mL (100 μL/well) can bind Anti-HA (B/Phuket/3073/2013) Monoclonal Antibody, Mouse IgG with a linear range of 0.5-63 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  0.1 M Sodium citrate, pH5.5

• Additives:

  Trehalose

• Molecular Weight:

  64.1 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  100ug*1

• Species:

  Influenza