Detergent-Free Total Protein Isolation Kit

CAT:

1111-30300

Size:

25 Preparations

Price:

Ask

For price, please contact [email protected]

Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Dry Ice Shipment: No

Detergent-Free Total Protein Isolation Kit - image 1

Detergent-Free Total Protein Isolation Kit - image 2

Detergent-Free Total Protein Isolation Kit

Description: Simple protocol that is free of detergents Fast processing time Process a variety of inputs Compatible with mass spectrometry and NMR spectroscopy Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix This kit provides a fast and simple procedure for the isolation of total proteins from tissue, bacteria, yeast or mammalian cells without the use of SDS, Triton® X-100 and other detergents. Detergents are extensively used to prepare protein samples; however, these detergents have undesirable effects on downstream analysis. These effects include extraneous peaks in mass spectrometry, artifacts with chromatography and electrophoresis, interference with microinjection into cells and interference with protein immunization. The Detergent-Free Total Protein Isolation Kit maintains high protein recovery and yields proteins that are 100% detergent-free. Purification is based on using Norgen’s proprietary resin together with Lysis Solution, followed by protein filtration using a filter column (provided). The purified proteins can be used in a number of downstream applications including mass spectrometry, SDS-PAGE, isoelectric focusing, NMR spectroscopy and more. Each Lysis Tube is able to process up to 50 mg of tissue, 1010 bacterial cells, 109 yeast cells or 107 mammalian cells. Preparation time for 12 samples is less than 10 minutes.
UNSPSC: 41106510
HS Code: 3822.19.00.80
Dot Regulated: false
Weight: 2
Length: 6
Storage Conditions: RT
Width: 10
Height: 6
Shelf Life: 2 years
Hazardous Volume: 0
Non Hazardous Volume: 0
Cubic Feet: 0.20833333333333334

Datasheet

Related Products

Cat	Name
30300	Detergent-Free Total Protein Isolation Kit
22200	ProteoSpin Total Protein Concentration Detergent Clean-Up And Endotoxin Removal Maxi Kit
22800	ProteoSpin Total Protein Concentration Detergent Clean-Up and Endotoxin Removal Mini Kit
17240	Total RNA Purification Kit
17270	Total RNA Purification Kit
17250	Total RNA Purification Kit
17200	Total RNA Purification Kit
37500	Total RNA Purification Kit
68803	Total Nucleic Acid Preservation Tube
27750	Soil Total RNA Purification Kit

Alternative Products

Cat	Name
P505S	3-min Detergent-free Total Protein Extraction Kit (Animal Cells)
P506L	3-min Detergent-free Total Protein Extraction Kit (Animal Tissues)
P505	3-min Detergent-free Total Protein Extraction Kit (Animal Cells)
P505L	3-min Detergent-free Total Protein Extraction Kit (Animal Cells)
P506	3-min Detergent-free Total Protein Extraction Kit (Animal Tissues)
P506S	3-min Detergent-free Total Protein Extraction Kit (Animal Tissues)
P510L	8-min Plant Tissue Detergent-free Total Protein Extraction Kit
SN-006	Minute ™ Detergent-Free Total Protein Extraction Kit for Animal Cells/Tissues
P201	Detergent-free Exosomal Protein Extraction Kit
P524-20	Detergent-Free Nuclei Isolation Kit