E. coli O157:H7 Quantified Bacterial Standard

E. coli O157:H7 Quantified Bacterial Standard

• Description: Quantified standard to be used as a positive control or PCR quantification standard Vigorously quantified using multiple methods Includes partial sequence of STX-1 subunit A, and STX-2 subunit A genes (please refer to product insert for more details) Compatible with E. coli O157:H7 TaqMan PCR Detection Kit and E. coli O157:H7 TaqMan PCR Detection Probe/Primer and Control Set E. coli O157:H7 is a rod-shaped, gram negative bacterium. It is an enterohemorrhagic strain of the common E. coli bacterium and infection by the O157:H7 strain is commonly associated with hemorrhagic colitis. E. coli O157:H7 is recognized by its somatic (cell wall) antigen (O157) and its flagella antigen (H7). In addition, E. coli O157:H7 is known to produce Shiga-like toxins, which cause severe symptoms. While most patients can recover from the infection, up to 15% of the patients may develop hemolytic uremic syndrome, a type of kidney failure that could be fatal. Infection of E. coli O157:H7 usually results from consumption of poorly prepared food including undercooked meat (particularly ground beef), untreated water or raw unpasteurized milk. Norgen’s E. coli O157:H7 Quantified Bacterial DNA Standards are cloned fragments of the two Shiga-like toxin regions purified using Norgen’s sample preparation technology. They include partial sequences of STX-1 subunit A gene and STX-2 subunit A gene. Please refer to Appendix A for sequence information. The purified DNA is quantified vigorously using multiple methods including spectrophotometry, gel densitometry and real-time PCR. It is intended to be used as positive controls or PCR quantification standards for E. coli O157:H7. This product is compatible with Norgen Biotek's E. coli O157:H7 Taqman PCR Detection Kit (Cat. TM41350) and E. coli O157:H7 Taqman PCR Detection Probe/Primer and Control Set (Cat. TM41310).
• CAS Number: 9007-83-4
• UNSPSC: 41106310
• HS Code: 3822.19.00.80
• Dot Regulated: true
• Weight: 28
• Length: 13
• Storage Conditions: Dry Ice -80°C -20°C
• Width: 15
• Height: 12
• Hazardous Volume: 0
• Non Hazardous Volume: 0
• Cubic Feet: 1.3541666666666667