MEF2B Antibody

• Description:

  The myocyte enhancer factor-2 (MEF-2) family of transcription factors associate with co-repessors or co-activators to regulate development and function of T cells, neuronal cells, and muscle cells. Four family members, termed MEF-2A, -2B, -2C, and -2D, arise from alternatively spliced transcripts. These members bind as homo- and heterodimers to the MEF-2 site in the promoter region of affected genes. Differential regulation in the expression of the four transcripts implies functional distinction for each during embryogenesis and development. The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including MyoD, myogenin, Myf-5, and MRF4, are one class of identified factors. The MEF-2 family represents a second class of DNA binding regulatory proteins. Each of these proteins binds to the MEF-2 target DNA sequence present in the regulatory regions of many muscle-specific genes.

• Specifications:

  Western blot: 1-2 µg/mL, Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL

• UniProt:

  Q02080

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human MEF2B protein was used as the immunogen for the MEF2B antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a

• Clone:

  PCRP-MEF2B-2F9

• Applications:

  WB, FACS, IF

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Aliquot the MEF2B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This MEF2B antibody is available for research use only.

• Storage Conditions:

  Aliquot the MEF2B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the MEF2B antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nucleus

• Image Legend:

  Immunofluorescent staining of PFA-fixed human HeLa cells using MEF2B antibody (green, clone PCRP-MEF2B-2F9) and phalloidin (red) .