Decapping Protein 2 Antibody / DCP2 / m7GpppN-mRNA hydrolase

• Description:

  The major pathway of eukaryotic mRNA decay involves deadenylation-dependent decapping followed by 5exonucleolytic degradation. Human decapping enzyme 2 (hDcp2) is an mRNA decapping enzyme which contains intrinsic decapping activity. In nonsense-mediated decay, the human decapping complex, made up of hDcp1 and hDcp2, may be recruited to mRNAs containing premature termination codons by nonsense-mediated decay factor (Upf) proteins. The decapping activator complex (Lsm1p-7p) is also involved in the recruitment of the decapping complex, indicated by data showing that Lsm1p-7p enhances the co-immunoprecipitation of the complex with mRNA. Dcp2 specifically hydrolyzes methylated capped RNA to release m7GDP, thereby aiding in mRNA degradation. Both Dcp1 and Dcp2 co-localize in the cytoplasm, which is consistent with their role in mRNA decay

• UniProt:

  Q8IU60

• Host:

  Mouse

• Immunogen:

  Recombinant full-length human protein was used as the immunogen for the Decapping Protein 2 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a

• Applications:

  FACS, IF

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the Decapping Protein 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Decapping Protein 2 antibody is available for research use only.

• CAS Number:

  9007-83-4