TDRKH Antibody

• Description:

  Participates in the primary piRNA biogenesis pathway and is required during spermatogenesis to repress transposable elements and prevent their mobilization, which is essential for the germline integrity. The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Required for the final steps of primary piRNA biogenesis by participating in the processing of 31-37 nt intermediates into mature piRNAs. May act in pi-bodies and piP-bodies by transferring piRNA precursors or intermediates to or between these granules.

• Specifications:

  Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  Q9Y2W6

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human TDRKH protein was used as the immunogen for the TDRKH antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b

• Clone:

  PCRP-TDRKH-1H2

• Applications:

  FACS, IF, WB, IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the TDRKH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This TDRKH antibody is available for research use only.

• Storage Conditions:

  Aliquot the TDRKH antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the TDRKH antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasm

• Image Legend:

  Immunofluorescent staining of PFA-fixed human HeLa cells using TDRKH antibody (green, clone PCRP-TDRKH-1H2) and phalloidin (red) .