Recombinant PSA Antibody / Prostate Specific Antigen

• Description:

  Prostate-specific antigen (PSA) is a single-chain glycoprotein of 237 amino acids containing approximately 8% carbohydrate. It is a serine protease produced almost exclusively by prostatic epithelial cells. Immunohistochemically PSA is expressed in the highly specialized apically-superficial layer of female and male secretory cells of the prostate gland, and is readily demonstrated in adenocarcinomas of the prostate in about 99% of the cases. There is a correlation between malignancy grade and intensity of staining, high grade carcinomas displaying weaker expression. About 1% of poorly differentiated carcinomas have been negative for PSA. Due to the high specificity of PSA for prostatic glandular epithelium, it is very useful in identifying prostatic carcinoma in the prostate and in the adjacent organs often affected by epithelial malignancies, i.e. rectum and urinary bladder. PSA may be used in a panel together with NKX3.1 and Prostein, which are at least as sensitive and slightly more specific than PSA.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  P07288

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 150-250 of Prostate-specific antigen protein was used as the immunogen for the recombinant PSA antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG

• Clone:

  KLK3/4602R

• Type:

  Recombinant

• Applications:

  IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the recombinant PSA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This recombinant PSA antibody is available for research use only.

• Storage Conditions:

  Aliquot the recombinant PSA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the recombinant PSA antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Location:

  Secreted

• Image Legend:

  IHC staining of FFPE human prostate carcinoma tissue with recombinant PSA antibody (clone KLK3/4602R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.