Recombinant CD5 Antibody

CAT: 0800-V9516-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#:0800-V9516-100UGSize:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The CD5 antigen is a transmembrane glycoprotein expressed on the surface of practically all mature human T-cells (about 10% of CD4+ T-cells being CD5 negative) . In immature (CD34+) T-cells, CD5 is weakly expressed, the intensity of expression increasing with maturation. CD5 is also expressed in a small subset of normal human B-cells (20% of B-cells in the peripheral blood, scattered cells in the lymph node mantle zone) . The CD5+ cells are probably involved in B-T interaction and their ligand is CD72 which is expressed on all B cells. It appears that CD5+ B-cells on activation primarily produce IgM. They also produce more autoantibodies than normal CD5 negative B-cells. Thus, the CD5+ B-cell population is expanded in rheumatoid arthritis and systemic lupus erythematosus. The CD5 antibody reacts with CD5 expressed on B and T cells, and may be a useful aid for the classification of B and T-cell malignancies. This includes B-cell chronic lymphoid leukemia (B-CLL), B-cell small lymphocytic lymphoma (B-SLL), mantle cell lymphoma (MCL) and T-cell lymphoma and leukemia.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P06127
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 450-495 from the intracellular region of human CD5 was used as the immunogen for the recombinant CD5 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
C5/4561R
Type
Recombinant
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the recombinant CD5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant CD5 antibody is available for research use only.
Storage Conditions
Aliquot the recombinant CD5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant CD5 antibody should be determined by the researcher.
Location
Cell Surface
Image Legend
IHC staining of FFPE human tonsil tissue with recombinant CD5 antibody (clone C5/4516R) at 2ug/ml in PBS for 30min RT. Strong surface staining observed. Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Product Datasheet
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