Bcl6 Antibody

• Description:

  Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL) . The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large (LH) cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma.

• Specifications:

  Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL

• UniProt:

  P41182

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant human BCL6 protein was used as the immunogen for the Bcl6 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b

• Clone:

  PCRP-BCL6-1D3

• Applications:

  FACS, IF

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the Bcl6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Bcl6 antibody is available for research use only.

• Storage Conditions:

  Aliquot the Bcl6 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the Bcl6 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nucleus, Cytoplasm

• Image Legend:

  Immunofluorescent staining of PFA-fixed human MCF-7 cells using Bcl6 antibody (green, clone PCRP-BCL6-1D3) and phalloidin (red) .