Daxx Antibody

• Description :

  This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants.

• Specifications :

  Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt :

  Q9UER7

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Recombinant full-length human DAXX protein was used as the immunogen for the Daxx antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2b

• Clone :

  PCRP-DAXX-5G11

• Applications :

  FACS, IF, IHC-P

• Purity :

  Protein A/G affinity

• Format :

  Purified

• Buffer :

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution :

  Aliquot the Daxx antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This Daxx antibody is available for research use only.

• Storage Conditions :

  Aliquot the Daxx antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes :

  Optimal dilution of the Daxx antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Location :

  Nucleus, Cytoplasm

• Image Legend :

  IHC staining of FFPE human thyroid tissue with Daxx antibody (clone PCRP-DAXX-5G11) at 2ug/ml. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.