RET Antibody / c-RET

• Description:

  The Ret proto-oncogene is structurally related to the growing family of tyrosine kinase transmembrane receptors and is involved in GDNF signaling. RET expression is reported in several regions of the central nervous system; in the developing cranial nerve ganglia and a subset of cells within dorsal root ganglia, in motor neurons in the spinal cord and hindbrain, in neuro-retina and the growing tips of the renal collecting ducts in developing kidney. Alterations in the RET gene are associated with diseases including papillary thyroid carcinoma, multiple endocrine neoplasia (type 2A and 2B), familial medullary thyroid carcinoma, and a congenital developmental disorder known as Hirschsprung s disease.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  P07949

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A recombinant fragment (within amino acids 702-848) of human RET protein was used as the immunogen for the RET antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2c κ

• Clone:

  RET/8786

• Applications:

  IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the RET antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This RET antibody is available for research use only.

• Storage Conditions:

  Aliquot the RET antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the RET antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell membrane, cytoplasm

• Image Legend:

  IHC staining of FFPE human placental tissue with Purified RET antibody (clone RET/8786) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.