Colipase Antibody / CLPS

• Description:

  The lipase gene family belongs to one of the most robust genetic superfamilies found in living organisms, which includes esterases and thioesterases. Members of the AB hydrolase subfamily include Hepatic Lipase (HL), Endothelial Lipase (EL), Lipoprotein Lipase (LPL), Pancreatic Lipase (PL), Gastric Lipase (GL) and LCAT. These family members play a crucial role in the metabolism of lipids. Pancreatic lipase, also designated pancreatic triacylglycerol acyl hydrolase, is important for dietary fat absorption as it hydrolyses triglycerides into diglycerides, monoglycerides and free fatty acids. Colipase, also known as CLPS or pancreatic colipase preproprotein, is a 112 amino acid secreted protein that functions as a cofactor of pancreatic lipase. Necessary for dietary lipid hydrolysis and localizing to pancreatic acinar cells, colipase allows pancreatic lipase to anchor itself to the lipid-water interface of lipid micelles, thereby preventing intestinal bile salts from washing it off.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

• UniProt:

  P04118

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A recombinant fragment (within amino acids 1-112) of human CLPS protein was used as the immunogen for the Colipase antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  CLPS/9027

• Applications:

  IHC-P, WB

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the Colipase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Colipase antibody is available for research use only.

• Storage Conditions:

  Aliquot the Colipase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the Colipase antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Secreted

• Image Legend:

  Western blot testing of human pancreas tissue lysate with Colipase antibody (clone CLPS/9027) . Predicted molecular weight ~12 kDa.