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CLPS Antibody / Colipase

CAT: 0800-V5410-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V5410-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The lipase gene family belongs to one of the most robust genetic superfamilies found in living organisms, which includes esterases and thioesterases. Members of the AB hydrolase subfamily include Hepatic Lipase (HL), Endothelial Lipase (EL), Lipoprotein Lipase (LPL), Pancreatic Lipase (PL), Gastric Lipase (GL) and LCAT. These family members play a crucial role in the metabolism of lipids. Pancreatic lipase, also designated pancreatic triacylglycerol acyl hydrolase, is important for dietary fat absorption as it hydrolyses triglycerides into diglycerides, monoglycerides and free fatty acids. Colipase, also known as CLPS or pancreatic colipase preproprotein, is a 112 amino acid secreted protein that functions as a cofactor of pancreatic lipase. Necessary for dietary lipid hydrolysis and localizing to pancreatic acinar cells, colipase allows pancreatic lipase to anchor itself to the lipid-water interface of lipid micelles, thereby preventing intestinal bile salts from washing it off.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL
UniProt
P04118
Host
Mouse
Reactivity
Human
Immunogen
A recombinant fragment (within amino acids 1-112) of human CLPS protein was used as the immunogen for the CLPS antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
CLPS/9021
Applications
IHC-P, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the CLPS antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This CLPS antibody is available for research use only.
Storage Conditions
Aliquot the CLPS antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the CLPS antibody should be determined by the researcher.
Location
Secreted
Image Legend
Western blot testing of human pancreas tissue lysate with CLPS antibody (clone CLPS/9021) . Predicted molecular weight ~12 kDa.

UniProtKB · P04118

Colipase

COL_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P04118
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CLPS
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q5T9G7, Q5U809
Protein keywords

Disease

Diabetes mellitus

Biological process

DigestionLipid degradationLipid metabolism

Technical term

Direct protein sequencingProteomics identificationReference proteome

PTM

Disulfide bond

Cellular component

Secreted

Domain

Signal

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