Recombinant IL-7 Antibody

• Description:

  Interleukin-7 (IL-7) was originally described as a factor capable of inducing in vitro proliferation of pre-B cells from marrow cultures. The IL-7 gene encodes a protein 177 amino acids in length. IL-7 exerts its biological function through the IL-7 receptor which is expressed on pre-B cells, thymocytes and bone marrow-derived macrophages. The IL-7 receptor is composed of an IL-7 receptor specific chain and the IL-2 receptor ? chain common to the IL-2, IL-4, IL-7, IL-9 and IL-15 receptors. IL-7 stimulation leads to the activation of Janus tyrosine kinase family members JAK1 and JAK3. Other studies have shown that in T cells, the IL-7 receptor-specific chain associates with the Src kinases family Lck and Fyn. IL-7 induces phosphorylation of Insulin receptor substrate-1 (IRS-1) and Insulin receptor substrate-2 (IRS-2), originally called 4PS.

• Specifications:

  Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-3 µg/mL

• UniProt:

  P13232

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A recombinant fragment (within amino acids 27-177) corresponding to the mature human IL7 protein was used as the immunogen for the recombinant IL-7 antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG κ

• Clone:

  IL7/7053R

• Type:

  Recombinant

• Applications:

  FACS, IF

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the recombinant IL-7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This recombinant IL-7 antibody is available for research use only.

• Storage Conditions:

  Aliquot the recombinant IL-7 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the recombinant IL-7 antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Location:

  Secreted

• Image Legend:

  SDS-PAGE analysis of purified, BSA-free recombinant IL-7 antibody (clone IL7/7053R) as confirmation of integrity and purity.