Recombinant HER-2 Antibody / ErbB2

• Description:

  C-erbB-2/HER-2 is a member of the EGFR family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  P04626

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A recombinant fragment (within amino acids 1155-1255) of human HER-2/ERBB2 protein was used as the immunogen for the recombinant HER-2 antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  RERBB2/9401

• Type:

  Recombinant

• Applications:

  IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the recombinant HER-2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This recombinant HER-2 antibody is available for research use only.

• Storage Conditions:

  Aliquot the recombinant HER-2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the recombinant HER-2 antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Location:

  Cell Surface

• Image Legend:

  IHC staining of FFPE human breast carcinoma tissue with recombinant HER-2 antibody (clone rERBB2/9401) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.