MPHOSPH9 Antibody / M-phase phosphoprotein 9

• Description:

  MPHOSPH9, also known as M-phase phosphoprotein 9, has 2 isoforms, a 1,031 amino acid isoform 1 that is 116 kDa and a 1,028 amino acid isoform 2 that is 116 kDa, localizes to the distal and proximal end of centriole pairs in duplicated centrosomes, and in ciliated cells, localizes to the distal and proximal end of daughter centriole and proximal of the mother centriole but not in the distal end of the mother centriole. Little is known about its function. Studies on this protein have shown a relationship with the following diseases and disorders: corneal ulcer, rosacea, multiple sclerosis, Fanconi's anemia, anemia, and neuronitis. This protein has also shown an interaction with YWHAG, USP11, SVIL, YWHAZ, WNK1, and UBC in M phase of mitotic cell cycle pathways.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

• UniProt:

  A0A8I5IG87

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  An E.coli-derived human recombinant protein (S49-Q1059) was used as the immunogen for the MPHOSPH9 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IF, FACS, ELISA

• Purity:

  Antigen affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the MPHOSPH9 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This MPHOSPH9 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the MPHOSPH9 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the MPHOSPH9 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  Immunofluorescent staining of FFPE human HeLa cells with MPHOSPH9 antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.