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RPB2 Antibody / POLR2B

CAT: 0800-RQ8383Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ8383Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
DNA-directed RNA polymerase II subunit RPB2 is an enzyme that in humans is encoded by the POLR2B gene. This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL
UniProt
P30876
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An E.coli-derived human recombinant protein (E49-Q1145) was used as the immunogen for the RPB2 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF, FACS, ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the RPB2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This RPB2 antibody is available for research use only.
Storage Conditions
After reconstitution, the RPB2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the RPB2 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Immunofluorescent staining of FFPE human A549 cells with RPB2 antibody (red) and Beta Tubulin mAb (green) . HIER: steam section in pH6 citrate buffer for 20 min.

UniProtKB · P30876

DNA-directed RNA polymerase II subunit RPB2

RPB2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P30876
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
POLR2B
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
2.7.7.6
Processing
—
Secondary accessions
A8K1A8, Q8IZ61
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Cellular component

DNA-directed RNA polymeraseNucleus

Molecular function

HydrolaseNucleotidyltransferaseRNA-directed RNA polymeraseTransferase

Ligand

MagnesiumMetal-bindingZinc

PTM

MethylationPhosphoprotein

Biological process

Transcription

Domain

Zinc-finger
Product Datasheet
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