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NPEPPS Antibody

CAT: 0800-RQ8367Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ8367Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Puromycin-sensitive amino peptidase also known as cytosol alanyl aminopeptidase or alanine aminopeptidase (AAP) is an enzyme that in humans is encoded by the NPEPPS gene. This gene encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P55786
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An E.coli-derived human recombinant protein (Y532-A896) was used as the immunogen for the NPEPPS antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS, Direct ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the NPEPPS antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This NPEPPS antibody is available for research use only.
Storage Conditions
After reconstitution, the NPEPPS antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the NPEPPS antibody should be determined by the researcher.
Location
Nuclear, cytoplasmic
Image Legend
IHC staining of FFPE human testicular germ cell tumor tissue with NPEPPS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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