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MIS12 Antibody

CAT: 0800-RQ8348Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ8348Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Protein MIS12 homolog is a protein that in humans is encoded by the MIS12 gene. Mis12 complex is composed of four subunits, Protein MIS12 homolog, Polyamine-modulated factor 1, Kinetochore-associated protein DSN1 homolog, and Kinetochore-associated protein NSL1 homolog (UniProt: Q9H081, Q6P1K2, Q9H410, Q96IY1, respectively) that are encoded by genes known as MIS12 (Gene ID: 79003), PMF1 (Gene ID: 100527963), DSN1 (Gene ID: 79980), and NSL1 (also known as C1orf48, DC31, DC8, MIS14) (Gene ID: 25936) in human. The MIS12 complex is a protein interaction hub for outer kinetochore assembly. This complex acts as the primary microtubule-binding interface at kinetochores and provides a platform to recruit regulatory proteins. In human Mis12 complex subunits are shown to localize with centromere protein A (CENP-A) at inner kinetochores and internally to Ndc80 at outer kinetochores. Mis12 complex plays an essential role in chromosome segregation in vertebrates and contributes to mitotic kinetochore assembly. Reduced levels of Mis12 complex proteins are shown to result in chromosome alignment defects in both human and chicken cells, but spindle bipolarity is not disturbed.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL
UniProt
Q9H081
Host
Rabbit
Reactivity
Human
Immunogen
An E.coli-derived human recombinant protein (M1-K187) was used as the immunogen for the MIS12 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF, FACS, ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the MIS12 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This MIS12 antibody is available for research use only.
Storage Conditions
After reconstitution, the MIS12 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the MIS12 antibody should be determined by the researcher.
Image Legend
Immunofluorescent staining of FFPE human U-2 OS cells with MIS12 antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

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