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NUP133 Antibody

CAT: 0800-RQ8319Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ8319Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Nuclear pore complex protein Nup133, or Nucleoporin Nup133, is a protein that in humans is encoded by the NUP133 gene. The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Immunoprecipitation: 2ug/500ug of lysate, Direct ELISA: 0.1-0.5 µg/mL
UniProt
Q8WUM0
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An E.coli-derived human recombinant protein (Q228-I1156) was used as the immunogen for the NUP133 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF, IP, Direct ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the NUP133 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This NUP133 antibody is available for research use only.
Storage Conditions
After reconstitution, the NUP133 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the NUP133 antibody should be determined by the researcher.
Image Legend
Immunofluorescent staining of FFPE human A549 cells with NUP133 antibody (green) and Beta Tubulin mAb (red) . HIER: steam section in pH6 citrate buffer for 20 min.

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